BCF297 Laser Scanning Confocal Microscopy
ʻO BCF297 kahi microscope confocal scanning laser hou i hoʻokuʻu ʻia, hiki ke hoʻokō i ka nānā pono kiʻekiʻe a me ka nānā pono. Hiki ke hoʻohana nui ʻia i ka morphology, physiology, immunology, genetics a me nā māla ʻē aʻe. He hoa kūpono no ka noiʻi biomedical ʻokiʻoki.
Nā hiʻohiʻona
1. Kiʻekiʻe hōʻailona-a-leo ratio.
Hiki ke hāʻawi i nā kiʻi fluorescence me ke kiʻekiʻe kiʻekiʻe kiʻi confocal imaging alahele hiki ke hāʻawi i nā kiʻi fluorescence me ke kiʻekiʻe kiʻekiʻe kiʻekiʻe o ka hōʻailona-a-noise ratio ʻoiai ma lalo o ka fluorescence nāwaliwali.
2. Kiʻi maikaʻi loa.
Laulā laula, lens aperture helu kiʻekiʻe, kūpono no ke kiʻi ʻana i nā ʻano laʻana confocal.
3. Maʻalahi e hoʻohana.
Kiʻi uila piha, hoʻolālā maikaʻi ʻia o ka pilina pili kanaka-kamepiula, e ʻae iā ʻoe e hana i kahi hana me ka maʻalahi i ka wā o ka pana ʻana.
4. Pūnaehana hoʻokele kaʻa a pau.
Hoʻohana ka Z-axis o BCF297 laser confocal microscope i kahi mīkini kaʻa, hiki ke hoʻololi koke i ke kiʻekiʻe Z-axis e like me ke kiʻi maoli. AF One-button autofocus, hoʻopau i ka pono no ka hoʻoponopono ʻana i nā ʻanuʻu a hoʻomaikaʻi i ka hana pono.
Hiki i nā pihi mana i hoʻohui ʻia ma nā ʻaoʻao ʻelua o ke kino ke hoʻomaopopo i ka hoʻololi wikiwiki a i ʻole ka hoʻololi ʻana o ka condenser, ka ʻōlinolino, ka lens pahuhopu, ka turntable film attenuation, a me ka turntable fluorescence, e hoʻomaikaʻi ai i ka maʻalahi o ka hana.
5. Optics Kiʻekiʻe no ke Kiʻi Confocal.
(1) ʻO ka hoʻolālā pinhole kiʻi kūʻokoʻa: e hōʻemi i ka hoʻopili ʻia e ka neʻe ʻana o nā mea a hoʻomaikaʻi i ka ratio signal-to-noise a me ka hoʻonā axial o ke kiʻi.
(2) Pūnaehana ʻike mana: ʻāpana ʻike kiʻekiʻe (QE≥45%@500nm kiʻekiʻe), hiki iā ia ke hoʻopiha pono i nā kiʻi confocal fluorescence multi-colored me ka maʻalahi a me ka wikiwiki.
6. ʻElua pūʻulu o nā lens pahuhopu koho.
ʻO BCF297 laser scanning confocal microscope he ʻelua pūʻulu o nā lens i koho ʻia, apochromatic (2X-100X) a me super-apochromatic (10X-100X), me kahi ākea o ka uhi hoʻonui, kūpono no ka microscopy noiʻi holomua a me ka pana kiʻi microscopic. Hoʻomaikaʻi hou ka puka helu nui i ka hoʻonā.
Pahuhopu APO Plan Infinite
Pahuhopu ʻo Super APO Plan Infinite
7. Nā polokalamu kūikawā no ka laser confocal.
Hoʻopiha ʻia ka polokalamu no kēia microscope confocal me nā hiʻohiʻona ikaika. Kākoʻo ʻo BCF297 i ke kiʻi kiʻi hoʻokahi a i ʻole multi-channel 2D (XY), 3D imaging (XYZ), 4D imaging (XYZT) a me ka nānā ʻana i nā pūnaewele lehulehu. Hiki ke hana ʻia ke kiʻi kiʻi, photobleaching a me photostimulation i ka ROI (Region of Interest) i wehewehe ʻia e ka mea hoʻohana, Z-Stack imaging, humuhumu kiʻi nui, ka hoʻoponopono ʻana i ka pālākiō, ka hana kānana, ka hoʻopaʻa ʻana i ka ʻikepili, etc.
Hōʻike
| 'ikamu | Hōʻike | BCF297 | |
| Puna kukui laser | Laser | 405nm/50mW, 488nm/50mW, 561nm/50mW, 640nm/40mW | ● |
| Mana laser | ʻO ka modulation pololei a me ka modulation acousto-optic, hiki ke hoʻoponopono pololei ʻia nā hoʻololi a me ka ikaika a pau, a hiki ke komo i ka mokuʻāina inā ʻaʻole hoʻohana ʻia no ka manawa pōkole. | ● | |
| ʻĀpana kiʻi | Unite scanning | Kālua-axis XY kiʻekiʻe-wikiwiki scan galvanometer | ● |
| Kahua ʻike 14mm×14mm (≥ 19) | ● | ||
| Ke nānā nei i nā pika 512×512～4096×4096 | ● | ||
| Ka manawa Pixel 0.5μs ~ 8μs | ● | ||
| ʻO ka māmā hiʻohiʻona maʻamau: 1fps (512×512, 2μs) ʻO ka māmā hoʻopale ʻana wikiwiki: 3fps (512×512, 0.5μs) | ● | ||
| Hoʻonui ʻia: 1-32X | ● | ||
| Pinhole | Ф30 / 40 / 50μm circular pinhole, aia ma waena o ka dichroic beam splitter a me ka scanning galvanometer, e hōʻoia i ka mālama ʻana o ka fluorescence hōʻiliʻili pono o ka ʻōnaehana ma 100% | ● | |
| Nā aniani Dichroic a me nā kānana | ʻEhā-kanal dichroic beam splitter: 405/488/561/640nm | ● | |
| 6-kūlana huila kānana kaʻa Hele mai maʻamau me ʻehā: 445nm/40, 530nm/43, 607nm/36 a me 685nm/40 | ● | ||
| ʻĀpana ʻike | MA PMT, QE ≥ 25%@500nm, 20%@600nm (PMT nui 1) | ● | |
| GaAsP PMT, QE ≥ 45%@500nm, 40%@600nm | ○ | ||
| ʻĀpana ʻike DIC | Me ka hana hoʻohālikelike ʻokoʻa, hiki iā ia ke ʻike i ke kiʻi "DIC-fluorescence". | ● | |
| ʻImi Noʻonoʻo Inverted Microscope | Pūnaehana Optical | Pūnaehana Optical Hoʻoponopono ʻia ʻo Infinity Chromatic Aberration | ● |
| Poʻo nānā | 20°-45° inclination hiki ke ho'ololi, ki'i ho'ohuli 'ia, pahu 'ike 'ike binocular infinity hinged, pae ho'oponopono mamao interpupillary: 50~76mm | ● | |
| Maka maka | Kiʻekiʻe maka kiʻekiʻe ākea kahua hoʻolālā eyepiece PL10X/22mm, adjustable diopter, micrometer | ● | |
| Leni pahuhopu | Nā Pahuhopu Apochromat Plan Infinity 4x, 10x, 20x, 40x, 60x, 100x | ● | |
| Pahuhopu Infinity Plan Super Apochromat 10x, 20x, 40x, 60x, 100x | ○ | ||
| Pākuʻi microscope | ʻO ke kūlana lima haʻahaʻa koʻikoʻi a me ka micro coaxial uila e kālele ana i ka mīkini, Z-axis stroke 10.5mm, pololei 1um; mua nui-nui LCD pā hōʻike panel, hoʻohui uila pihi mana ma ke kino, i hiki ke ike condenser, olinolino, pahuhopu lens, optical awa, a pela aku. Aia ma ka ʻaoʻao hema o ka fuselage, hoʻokaʻawale i ka ratio 100:0, 0:100; i kūkulu ʻia i loko o ka moku kaʻa hema, ka māhele māhele 0:100, 50:50, 100:0; hoʻokahi-layer/double-layer optical ala koho , e hāʻawi ana i lumi no ka hoʻonui ʻana i ka ʻōnaehana, a me ka hoʻololi ʻana i hoʻokahi kī o nā modula ala optical o kēlā me kēia papa e like me nā pono. Me ka Fluorescent Visor, 6-Position Motorized Converter (me DIC Slot) a me Glazed Port CTV Adapter Kit | ● | |
| Ke kahua | Paena mīkini lima, nui papa 300mm(X)×240mm(Y), laula neʻe 135mm(X)×85mm(Y) ʻO ke kahua uila, ka nui o ka papaʻaina ʻaʻole i emi iho ma mua o 260mm(X) × 153mm (Y), neʻe ka laulā 110mm (X) × 75mm (Y), me ka lima hana kūʻokoʻa; ʻO ka wikiwiki kiʻekiʻe 3mm/S, e hana hou i ka pololei o ka hoʻonohonoho ʻana ± 1um; Hoʻolako ʻia me ka ipu petri 35mm, nānā ʻāpana | ○ | |
| Condenser | Mea hoʻoheheʻe ʻia ʻo 7 puka, NA 0.55, WD 27mm; 3 puka no φ30mm (phase contrast), 4 puka no φ38mm (DIC); kākoʻo i ka māla mālamalama, ka ʻokoʻa o ka pae, ka nānā DIC (me ka hoʻonohonoho polarizer) | ● | |
| Pūnaehana kukui uila | ʻO 8-hole fluorescent turntable system, hiki i ka ʻōnaehana ke hoʻoholo i ke kūlana o ka turntable i kau ʻia ma luna a me lalo o ka papa; ia no hoi i ka uila pani pani, hiki ke ālai pono i ka fluorescent kumu kukui, me ka fluorescent kānana poloka aniani pūʻulu: B/G/UV, etc. | ● | |
| polokalamu hana
| Kiʻi kiʻi ʻana | Loaʻa kiʻi a me ka ʻōnaehana hoʻokele ʻokoʻa hana, hoʻololi mana uila piha o ke ala optical; hoʻololi ponoʻī o nā ʻāpana kamera a me nā hiʻohiʻona hiʻohiʻona; piha piha o ka nānā a me ka ROI scanning imaging; kiʻi kala hoʻokahi a i ʻole nā kala he nui 2D (XY), kiʻi 3D (XYZ), kiʻi 4D (XYZT) a me ka nānā ʻana i nā pūnaewele lehulehu. | ● |
| Ke kālailai ʻana | ʻO ka hoʻoili ʻana o ka colocalization fluorescence multi-color, fluorescence a me ka hoʻopili kiʻi ʻokoʻa (DIC) kiʻi kiʻi; calibration a hoʻohui i nā unahi; hana kānana; ʻO ka nānā ʻana i ka hana Z-Stack a me ka humuhumu kiʻi nui | ● | |
| Hooponopono ikepili | Hiki ke hoʻonohonoho ʻia nā ala hoʻopaʻa kiʻi ʻokoʻa, nā ʻano hoʻopuka kiʻi he nui, a hiki ke mālama ʻia nā ʻāpana kiʻi scanning. | ● | |
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