ʻO ka microscope fluorescence he ʻano microscope optical e hoʻohana ana i kahi kumu kukui ikaika nui e hoʻomālamalama i ka specimen a hoʻoulu i nā fluorochromes i ka hāpana. Hana ʻia ka hoʻomālamalama ʻana o ka specimen me kahi kumu kukui e hoʻokuʻu i ke kukui ultraviolet. Hoʻohana nui ʻia lākou i nā ʻoihana olaola, lāʻau lapaʻau a me nā ʻoihana.
Pehea e hana ai ka microscope fluorescence?
Hoʻohana ka microscope fluorescence i ka mercury a i ʻole ke kukui xenon e hana i ke kukui ultraviolet. Hele mai ke kukui i loko o ka microscope a paʻi i ke aniani dichroic - he aniani e hōʻike ana i hoʻokahi ʻano o nā lōʻihi hawewe a hiki ke hele i kahi ākea ʻē aʻe. Hōʻike ka aniani dichroic i ke kukui ultraviolet a hiki i ka specimen. ʻO kekahi mau hiʻohiʻona e lele maoli ana ma lalo o ke kukui ultraviolet no ka mea aia i loko o lākou nā mea fluorescent e like me ka chlorophyll. Inā 'a'ole 'olu'olu maoli ka mea ho'ohālike e 'ike 'ia, hiki ke ho'opa'a 'ia me nā mea 'olu'olu i kapa 'ia he fluorochromes.
He aha ke ʻano o ka microscope fluorescence?
1. Puna Māmā
ʻO nā kumu kukui maʻamau ka mercury, xenon, a me nā LED. Hāʻawi ʻo Mercury i ka maikaʻi o ka mālamalama no ka microscope fluorescence. Ke ulu nui nei nā LED no ka mea ʻoi aku ka liʻiliʻi o ke kumukūʻai ma mua o nā kumu ʻē aʻe a ʻoi aku ka liʻiliʻi o ka mana.
| Lapa Mercury | Ke kukui Xenon | LED | |
| Ka lōʻihi o ka nalu o ke kukui i hoʻokuʻu ʻia | 350-370 nm | 400nm～ 450nm | 400-700 nm |
| Ola (hola) | 200-300 | 400-600 | 10000 |
| Pōmaikaʻi | 1. Kiʻekiʻe Intensity Māmā, nā kumu kukui maʻamau. 2. Hoʻopuka ia i ka ultraviolet ikaika a me ka uliuli-violet e hoʻonāukiuki i nā ʻano fluorophores āpau. 3. Nā kiʻi ʻālohilohi a waihoʻoluʻu. | 1. Paa ka ikaika spectral. 2. Ka ikaika spectral ikaika i ka infrared a me ka waena-infrared. | 1. Maʻalahi e pani. 2. ʻAʻole pono e mahana. 3. Hiki ke hoʻololi a hoʻopau koke. 4. E hoʻomalu i ka ikaika kumu kukui. |
| Ka pōʻino | 1. He Ola Pokole. 2. Ka manawa pumehana lōʻihi. | 1. Pono ka pahu mana DC haʻahaʻa haʻahaʻa kūikawā no ka wela nui. 2. ʻOi aku ke kumukūʻai ma mua o ke kukui Mercury | 1. ʻOi aku ka nāwaliwali o ka kukui UV ma mua o ke kukui mercury. 2. ʻO kēlā me kēia nalu o ka mālamalama e koi i kahi LED kaʻawale ma muli o nā bandwidth haiki a lākou e hoʻopuka ai. |
Pākuʻi Fluorescent Mercury
Hoʻopili ʻia ʻo LED Fluorescent
2. Kānana Hoʻohauʻoli
Pono ka kānana excitation no ka hana ʻana o kahi microscope fluorescence. Hāʻalo ʻo ia i ke kukui o kahi hawewe pōkole, hiki ke hoʻopaʻa ʻia i ka wai kala fluorescent. Eia kekahi, pale ia i nā kumu ʻē aʻe o ke kukui hoihoi.
Kānana Hoʻohauʻoli(B,G,U,V)
3. Ke aniani Dichroic
ʻO ke aniani dichroic kahi ʻano kānana optical e hōʻike ana i ka mālamalama i kekahi mau nalu i ka wā e hoʻouna ana i nā mea ʻē aʻe. Hoʻohana ʻia ia i loko o nā microscopes fluorescence e hoʻokaʻawale i ka nalu hauʻoli a me ka hoʻokuʻu ʻana.
4. Kānana hoʻokuʻu
Holo ka kānana hoʻokuʻu wale i nā lōʻihi nalu i hoʻokuʻu ʻia e ka fluorophore a hoʻopaʻa i nā kukui makemake ʻole a pau ma waho o kēia pūʻulu - ʻoi aku ka nui o nā lōʻihi hawewe.
5. ʻO nā mea hoʻoluʻu fluorescent
ʻO nā mea hoʻoheheʻe fluorescent nā mea hoʻohuihui i loaʻa ka waiwai o ka fluorescence, kahi e hiki ai iā lākou ke hana i ke kiʻi fluorescent ma o ka hoʻokuʻu ʻana i nā kukui ʻōmaʻomaʻo ʻike ʻokoʻa loa ma hope o ka hauʻoli ʻana e ke kukui ultraviolet. ʻO nā mea waihoʻoluʻu fluorescent maʻamau; DAPI (49,6-diamidino-2-phenylindole), ʻalani acridine, auramine-rhodamine, Alexa Fluors, a i ʻole DyLight 488.
ʻEhia nā ʻano microscopes fluorescence?
1. ʻEpifluorescence Microscope pololei:
ʻO ia ke ʻano maʻamau o ka microscope fluorescence. Hana ʻia ka hauʻoli o ka fluorophore a me ka ʻike ʻana i ka fluorescence ma ke ala māmā like (ʻo ia hoʻi ma o ka pahuhopu). ʻO ka hapa nui o nā microscopes fluorescence, ʻoi aku ka nui o nā mea i hoʻohana ʻia i ka ʻepekema ola, no ka hoʻolālā epifluorescence.
BS-2081F Research Fluorescent Biological Microscope
2. ʻO ka Microscope Fluorescence Confocal:
Confocal Fluorescence Microscope: Hoʻohui kēia ʻano microscope fluorescence i ka nānā ʻana i ka laser me ka mālamalama fluorescent e hana i kahi kiʻi. Hiki ke hoʻohana ʻia i nā ʻano hana like ʻole, e like me ke aʻo ʻana i nā cell a me nā ʻiʻo, ka ʻike ʻana i nā protein a me nā mea ʻē aʻe i loko o nā cell, a me ke ana ʻana i ka mānoanoa o nā mea.
BS-2081F Research Fluorescent Biological Microscope
3. ʻO ka Microscope Fluorescence Inverted
Aia kēia ʻano kumu māmā o ka microscope a me ka condenser ma luna, e huli ana i lalo. Pono ke kihi o ka hoʻomālamalama ʻana ma 90 degere e pili ana i ka ʻili o ka specimen e nānā ʻia nei.
ʻO BS-2095F ʻAi ʻAi ʻAi ʻAi
He aha nā pōmaikaʻi a me nā pōʻino o nā microscopes fluorescence?
Pono:
- Hiki i ka nānā ʻana i nā cell i loko o nā mea ola me ka ʻole e hōʻino iā lākou
- Hāʻawi i nā kiʻi hoʻonā kiʻekiʻe me nā kala pololei
- Hāʻawi i ke aʻo ʻana i nā kaʻina ola i loko o nā cell
- E hoʻohana ʻia no ka ʻike ʻana i nā ʻano molekole like ʻole i loko o nā cell, e like me nā protein a i ʻole nā ʻakika nucleic.
Nā hemahema:
-ʻAe wale ia i ka nānā ʻana i nā hale kikoʻī i loko o kahi keʻena i hoʻopaʻa ʻia me ka dye fluorescent.
-ʻO ka photobleaching ma muli o ka excitation electron i ka wā o ke kaʻina hana o ka fluorescence hiki ke hoʻopili i nā molekole reactive o nā mea ʻulaʻula. ʻO ka hopena, hiki i nā mea hoʻoheheʻe reactive ke nalowale i ko lākou waiwai kemika o ka ikaika o ka hoʻokuʻu ʻana i ka fluorescence.
-E maʻalahi nā cell i ka hopena phototoxic ma hope o ka hoʻopaʻa ʻia ʻana me nā mea hoʻonaninani fluorescent, no ka mea, ʻo nā molekala fluorophore e komo i nā photon ikehu kiʻekiʻe mai ke kukui lōʻihi pōkole.
He aha nā mea hoʻohana o ka microscope fluorescence?
Hoʻohana nui ʻia nā microscope fluorescence i nā ʻano noiʻi a me ka noiʻi ʻana me ka biochemistry, cell biology, microbiology, immunology, a me ka lāʻau lapaʻau.
1. Ma ke kahua o ka biology, hiki i ka microscope fluorescence ke ʻike pololei a me ka kikoʻī o nā ʻāpana cellular a me submicroscopic cellular a me nā hana me ke kōkua o ka lepili dye fluorescent.
2. Ma ke kahua lapaʻau, hiki i ka microscope fluorescence ke hoʻohana i nā reagents fluorescent e ʻike ai i ka hele ʻana a me ka hāʻawi ʻana i nā maʻi bacteria a me nā maʻi, a i ʻole ke kōkua ʻana i ka hōʻailona ʻana i nā pahuhopu ʻokiʻoki e hoʻomaʻamaʻa i ke ʻoki.
3. Ma ke kahua o ka mineralogy, hoʻohana pinepine ʻia ka microscope fluorescence e aʻo i nā mea me nā waiwai fluorescence kūlohelohe, e like me ka asphalt, petroleum, lanahu, graphene oxide a me nā minerale ʻē aʻe.
4. Ma ka ʻepekema waiwai, hiki ke hoʻohana ʻia ka microscope fluorescence i ka ʻoihana textile a i ʻole ka ʻoihana pepa e kālailai i nā mea pili i ka fiber.
Ka manawa hoʻouna: Mar-14-2023