Ua hoʻololi ʻo Fluorescence microscopy i ko mākou hiki ke nānā a nānā i nā specimen biological, e ʻae iā mākou e komo i loko o ka honua paʻakikī o nā cell a me nā mole. ʻO kahi mea nui o ka microscopy fluorescence ke kumu māmā i hoʻohana ʻia no ka hoʻoulu ʻana i nā molekala fluorescent i loko o ka hāpana. I nā makahiki, ua hoʻohana ʻia nā kumu kukui like ʻole, kēlā me kēia me kona mau hiʻohiʻona kūʻokoʻa a me nā pono.
1. Ke kukui Mercury
Hoʻokumu ʻia ke kukui mercury kiʻekiʻe, mai 50 a 200 watts, me ka hoʻohana ʻana i ke aniani quartz a he spherical i ke ʻano. Aia kekahi nui o ka mercury i loko. I ka wā e hana ai, puka mai ka hoʻokuʻu ʻana ma waena o ʻelua mau electrodes, e hoʻoheheʻe ʻia ka mercury, a piʻi koke ke kaomi o loko o ka pōʻai. ʻO kēia kaʻina hana ma kahi o 5 a 15 mau minuke.
ʻO ka hoʻokuʻuʻiaʻana o ke kukui mercury kiʻekiʻe ka hopena mai ka weheʻana a me ka ho'ēmiʻana o nā molekele mercury i ka wā o ka hoʻokuʻu electrode, e alakaʻi ana i ka hoʻokuʻuʻana i nā photons māmā.
Hoʻopuka ʻo ia i nā kukui ultraviolet a me ka uliuli-violet kukui, e kūpono ana i nā mea hoʻonaninani like ʻole, ʻo ia ke kumu i hoʻohana nui ʻia ai i ka microscopy fluorescence.
2. Na kukui Xenon
ʻO kekahi kumu kukui keʻokeʻo maʻamau i hoʻohana ʻia i ka microscopy fluorescence ʻo ke kukui xenon. ʻO nā kukui Xenon, e like me nā kukui mercury, hāʻawi i kahi ākea ākea o nā lōʻihi hawewe mai ka ultraviolet a kokoke i ka infrared. Eia naʻe, ʻokoʻa lākou i kā lākou spectra excitation.
Hoʻopili nā kukui Mercury i kā lākou hoʻokuʻu ʻana i nā wahi kokoke i ka ultraviolet, uliuli, a me ka ʻōmaʻomaʻo, e hōʻoiaʻiʻo ana i ka hoʻokumu ʻana o nā hōʻailona fluorescent akā hele mai me ka phototoxicity ikaika. No laila, mālama ʻia nā kukui HBO no nā laʻana paʻa a i ʻole nā kiʻi fluorescence nāwaliwali. ʻO ka ʻokoʻa, ʻoi aku ka maʻalahi o nā kumu kukui xenon, e ʻae ana i ka hoʻohālikelike ʻana i ka ikaika ma nā lōʻihi nalu like ʻole. He mea maikaʻi kēia ʻano no nā noi e like me ke ana ʻana o ka calcium ion. Hōʻike pū nā kukui Xenon i ka hauʻoli ikaika ma kahi kokoke-infrared ākea, ʻoi aku ma kahi o 800-1000 nm.
Loaʻa i nā kukui XBO nā mea maikaʻi ma mua o nā kukui HBO:
① ʻOi aku ka like ʻole o ka ikaika spectral
② ʻOi aku ka ikaika o ka spectral i loko o ka infrared a me ka mid-infrared
③ ʻOi aku ka nui o ka ikehu, e maʻalahi ka hiki ʻana i ka puka o ka pahuhopu.
3. Nā LED
I nā makahiki i hala iho nei, ua puka mai kahi mea hoʻokūkū hou i ke aupuni o nā kumu kukui microscopy fluorescence: LED. Hāʻawi nā LED i ka pono o ka hoʻololi wikiwiki ʻana i nā milliseconds, e hōʻemi ana i nā manawa hōʻike hōʻike a hoʻonui i ke ola o nā laʻana maʻalahi. Eia kekahi, hōʻike ka kukui LED i ka pōʻino wikiwiki a pololei, e hōʻemi nui ana i ka phototoxicity i ka wā lōʻihi o nā hoʻokolohua kelepona ola.
Ke hoʻohālikelike ʻia i nā kumu kukui keʻokeʻo, hoʻopuka pinepine nā LED i loko o kahi kikoʻī hoʻohiwahiwa haʻahaʻa. Eia nō naʻe, loaʻa nā pūʻulu LED he nui, e ʻae ana i nā noi fluorescence multi-color, e hoʻolilo ana i nā LED i mea kaulana loa i nā hoʻonohonoho microscopy fluorescence hou.
4. Lasers Māmā Puna
ʻO nā kumu kukui laser he monochromatic a kuhikuhi ʻia, e hoʻolilo iā lākou i mea kūpono no ka microscopy kiʻekiʻe, me nā ʻenehana super-resolution e like me STED (Stimulated Emission Depletion) a me PALM (Photoactivated Localization Microscopy). Koho maʻamau ʻia ke kukui laser e hoʻohālikelike i ka lōʻihi o ka nalu hauʻoli i koi ʻia no ka fluorophore kiʻekiʻe, e hāʻawi ana i ke koho kiʻekiʻe a me ka pololei i ka excitation fluorescence.
ʻO ke koho ʻana i kahi kumu kukui microscope fluorescence e pili ana i nā koi hoʻokolohua kikoʻī a me nā hiʻohiʻona hiʻohiʻona. E ʻoluʻolu e kelepona mai iā mākou inā makemake ʻoe i ke kōkua
Ka manawa hoʻouna: Sep-13-2023